Protein_Domain

Part:BBa_K1362474:Design

Designed by: Jan Gleixner   Group: iGEM14_Heidelberg   (2014-10-09)


N terminal Gly insert for RFC[???] C intein constructs with two insertion sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As a sequence of length 0 could not be cloned as an oligo into an RFC[???] the sequence connects the ATG start codon of RFC[???] overhang A with overhang C by introducing 4 Gly codons.


Source

References